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KMID : 1123920110250040663
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Korean Journal of Oriental Physiology and Pathology
2011 Volume.25 No. 4 p.663 ~ p.668
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Protective Effect of Galgeun-Tang Against Induced Hepatotoxicity
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Oh Su-Young
Seo Sang-Hee
Lee Ji-Hye
Lee Ji-Seon
Ma Jin-Yeul
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Abstract
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Galgeun-tang (GGT) has been a great source for treating cold diseases in the folk medicine recipe. Carbon tetrachloride () is one type of hepatotoxin that can eventually cause liver injury. During the experiment, we first studied the protective effects of GGT against -induced hepatotoxicity. GGT was pretreated for 3 h, and 1% was added to mouse primary liver cells. After 4 h, ROS generation and expression of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)) were analyzed by FACS and real time PCR. Also, the activities of ALT and LDH were measured using cultured medium. The hepatic levels of TNF-alpha and iNOS, which are related to inflammation and stress response gene, HSP72 and HO-1 were analyzed by PCR or real time PCR. Liver tissues were analyzed by HE stain. From the observation, we discovered that GGT treatment protects -induced hepatotoxicity, and that GGT pretreatment decreases ROS generation, TNF-alpha and iNOS expression. However, gene expression of CAT, SOD, GPx, HSP72 and HO-1 were increased by GGT. These results lead to the conclusion that GGT has protective effects against -induced hepatotoxicity.
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KEYWORD
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Galgeun-tang, hepatotoxicity, carbon tetrachloride
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